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BIoanlysis - LEB

Course:

Biomedical Engineering

Curricular Unit (UC)

Bioanlysis

Mandatory  x
Optional  
Scientific Area EB Category  

Course category: B - Basic; C - Core Engineering; E - Specialization; P - Complementary.

Year: 2nd Semester: 2nd ECTS: 5.5 Total Hours: 140
Contact Hours T: 30 TP: 15 PL:15 S: OT:3
Professor in charge

 Manuel José de Matos

T - Lectures; TP - Theory and practice; PL - Lab Work; S - Seminar; OT - Tutorial Guidance.

  • Intended learning outcomes (knowledge, skills and competences to be developed by the students):

    After approval in the course, the student should:

    1. Know with depth the Analytical Methods used in the field of biomedical sciences;

    2. Know the basic principles of the equipment operation.

    3. Be able to select the most appropriate analysis method meeting the requirements of the analysis, the matrix of test compounds, equipment availability and associated costs;

    4. Know about sampling fundamentals.

    5. Implement and validate new analytical methods, specifically bioanalytical methods;

    6. Ensure and demonstrate the quality of the analytical results obtained;

  • Syllabus:

    1. Principles of Analytical Biochemistry.

    2. Introduction to Chemometrics and its application to Bioanalysis.

    3. Colorimetry, Fluorescence, Luminescence, Nephelometry, FTIR: Spectroscopic Methods.

    4. Electrochemical methods

    5. Chromatographic Methods: Liquid Chromatography, HPLC, Ion Exchange Chromatography, Affinity / Immunoaffinity and molecular exclusion.

    6. Methods of molecular recognition.

    7. Other methods used in bioanalysis: Mass Spectroscopy, Radiochemical methods, Structural analysis by diffraction of X-ray, NMR, ESR.

    8. Selection and validation of analytical methods.

  • . Evidence of the syllabus coherence with the curricular unit’s intended learning outcomes

    The syllabus follows the criteria used internationally in similar courses in biomedical sciences degrees. The connection between the methods of chemical analysis and its application to biomedical sciencesis is adequately supported by real examples.

    All objectives of the course are supported on one or more aspects of the program. An integrated content strategy with the integration of previous contents in the following points of the program is used. This strategy promotes the mainstreaming of subjects taught and links them to various and diverse aspects of bioanalysis.

  • Teaching methodologies (including assessment):

    Teaching methodologies:

    The teaching methodology is based on a theoretical and practical model.

    The classes follow the expository-interactive method, always accompanied by practical examples and with extensive use of the white board and the presentation of real cases in electronic format (Powerpoint, films, newspaper reports, etc.). Exercises addressing the theoretical concepts are solved.

    In practice classes, integrators and transversal examples of practical works will be realized.

    The discussed material is available in advance to the student.

    In the tutorial hours students complement their individual study clarifying the issues where they still have doubts.

    Continuous evaluation:

    Three Tests (T1..3), after each syllabus block.

    (T1..3)>=7.5

    NF = (T1+T2+T3)/3

    Practice Grade = NP

    Final Grade,  NF = 0.6xNT+ 0.4xNP

    NF >= 9.5

    Final exam evaluation:

    Final Exam (EF).

    NF = EF

    NF = 0.6xEF+ 0.4xNP

    NF>= 9.5

    Marks rounded to units. By defect, beneath five tenths, per excess, from five tenths.

  • . Evidence of the teaching methodologies coherence with the curricular unit’s intended learning outcomes

    The fundamental concepts introduced are applied and consolidated in classes.

    Lectures always include several practical examples which promote classroom discussion and easier assimilation of the theory as well as its connection with reality and with other courses in the MEQB. The exercises and practical works proposed allow students, individually or in group, to apply theoretical concepts to a wide variety of practical situations, and thus gain the confidence and skill to properly contextualize other situations.

    By this approach is also promoted the development of skills of contextual analysis and critical thinking skills.

    Learning outcomes are individually assessed by three written tests during the semester, allowing monitoring of the learning progression of the student.

    The practical classes are assessed through written reports and their discussion.

  • Main Bibliography

    1. Holme, D., Peck, H., “Analytical Biochemistry”, Prentice Hall, 3ª Ed., 1998.

    2. Gault, V., McClenaghan, N., “Understanding Bioanalytical Chemistry: Principles and Applications”, Wiley, 2009.

    3. Mikkelsen, S.R., Cortón, E., “Bioanalytical Chemistry”, John Wiley & Sons, 2006.

    4. Manz, A., Pamme, N., Lossifidis, D., “Bioanalytical Chemistry”, World Scientific Publishing Company, 2004.

    5. Gault, V., McClenaghan, N., “Understanding Bioanalytical Chemistry: Principles and Applications”, Wiley, 2009.

    6. Skoog, D.A., Holler, F.J., Crouch, S.R., “Principles of Instrumental Analysis”, Brooks Cole; 6ª Ed., 2006.

    7. Oliveira, A.G., “Bioestatística, Epidemiologia e Investigação”, LIDEL, 2009.